Food and Fermentation Industries >

2011 , Vol. 37 >Issue 02: 182 - 185

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2011.02.046

Food and Fermentation Industries

Determination of Amino Acids in Walnuts by Ultra Pefformanace Liquid Chromogray(UPLC) with Pre-column Derivation

  • Yang Jun ,
  • Wang Wen-hui ,
  • Li Yi ,
  • Wang Qi ,
  • Sun Xiao-dong ,
  • Zhu Yan-qin
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Online published: 2011-02-25

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Abstract

To establish an UPLC method in determination the amounts of Amino Acids in Walnuts.A Waters Acquity UPLC BEH C18 column(2.1 mm×150 mm,1.7μm)was applied,using AccQ.Tag Ultraa eluent A and AccQ.Tag Ultraa eluent B as the mobile phase,with a flow rate of 0.7 mL/min,and the column temperature 55℃.The calibration curve was linear in the range of 0.003~0.18 mg/mL of Amino Acids(r>0.999 4).The average recovery of Amino Acids was 98.5%.It has the advantage of simple,accuracy,and repeatable in the determination Amino Acids.

Key words： ultra performance liquid(UPLC); amino acids; kernel of walnut; quantitative analysis

Cite this article

Yang Jun , Wang Wen-hui , Li Yi , Wang Qi , Sun Xiao-dong , Zhu Yan-qin . Determination of Amino Acids in Walnuts by Ultra Pefformanace Liquid Chromogray(UPLC) with Pre-column Derivation[J]. Food and Fermentation Industries, 2011 , 37(02) : 182 -185 . DOI: 10.13995/j.cnki.11-1802/ts.2011.02.046

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