The Ophiopogon japonicus polysaccharide was hydrolyzed by bio-enzyme and the products were analyzed.The relative molecular weight of the Ophiopogon japonicus polysaccharide was 48347,which was purified by Sephadex G-100.The Ophiopogon japonicus polysaccharide was hydrolyzed by enzyme of Absidia sp.O8s,and the products were then isolated using Sephadex G-100 and Sephadex G-50 to generate three components: OP-1,OP-2 and OP-3.Their relative molecular weights were 32452,9231,1354,respectively.From HPLC,the purified Ophiopogon japonicus polysaccharide was mainly composed of glucose and fructose,then,OP-1,OP-2,OP-3 respectively consisted of fructose,fructose,glucose and fructose.