A two-phase process combined production of cellulosic ethanol and hydrogen from cornstalk was constructed in this paper.The effect of detoxification methods on the production of ethanol and hydrogen from acid-hydrolysis cornstalk were investigated.The results showed that using anion-exchange resins detoxification followed by evaporation,the maximal ethanol and hydrogen yields from cornstalk were 131.1 mg/g TS and 44.6 mL/g TS,respectively.Using acid hydrolysis method,the hemicellulose in cornstalk was degraded for ethanol production.The natural hydrogen-producing microorganism had the ability to directly degrade cellulose for hydrogen fermentation.