Radial flow chromatography(RFC) was a new technique of chromatography purification,and has a great potential in biotechnology.The deproteinization and decoloration of polysaccharide from Ganoderma lucidium by radial flow chromatography were studied.The optimal conditions were determined by single factor experiments and response surface experiments: sample concentration 10 mg/mL,sample volume 100 mL,sample flow-rate 2 mL/min and elution flow-rate 40 mL/min.The deproteinization rate,decoloration rate and polysaccharide recovery rate under the optional chromatography condition were 80.35%,88.52% and 85.06%,respectively.The purification efficiency of RFC was compared with sevag method,trichloroacetic acid method,activated carbon method and hydrogen perioxide method.The comparative results indicated that the purification by RFC were superior to other methods.None organic reagent in RFC treatment shows its advantages of high-efficiency,more economic,safe,and environmental friendly.