Food and Fermentation Industries >

2011 , Vol. 37 >Issue 03: 94 - 98

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2011.03.018

Food and Fermentation Industries

Study on the Fermentation for Fibrinolytic Enzyme from Pichia pastoris pk53

  • Jiao Long ,
  • Yu Hong-wei ,
  • Guo Run-fang ,
  • Bai Yu ,
  • Jia Ying-min
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Online published: 2011-03-25

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Abstract

The fermentation medium and conditions of the strain were studied via single factor test and orthogonal design.The optimal fermentation medium was determined as follows:glucose 1.5%,soypeptone 2.0%,KH2PO4 0.5%,MgSO4·7H2O 0.05%.The best fermentation conditions were:inoculating time 14 h,inoculating amount 1%,methanol supply 1.5%,medium volume 30 mL in 250 mL flask,initial pH 5.5.Under such conditions,the activity of fibrinolytic enzyme(corresponding to the unit of urokinase activity) was 429.06 U/mL,which was 8.88 times of the initial strain.

Key words： Pichia pastoris; fibrinolytic enzyme; fermentation; optimization

Cite this article

Jiao Long , Yu Hong-wei , Guo Run-fang , Bai Yu , Jia Ying-min . Study on the Fermentation for Fibrinolytic Enzyme from Pichia pastoris pk53[J]. Food and Fermentation Industries, 2011 , 37(03) : 94 -98 . DOI: 10.13995/j.cnki.11-1802/ts.2011.03.018

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