In this paper,different Fenjiu fermentation samples,Fen Daqu starter and environment samples were used for DNA extraction.Two different methods,the enzymatic-physical-chemical method and physical-chemical method,were implemented to accomplish the stated goal,and the former method led to quality DNA with high purity.The follow-up experiment showed that the DNA obtained through the enzymatic-physical-chemical method allowed full reflection of the diversity of the microbial community from every different sample during the production and fermentation of Fenjiu.