The interaction between hydrogen bonding in 40% and 60% volume fraction of ethanol-water spirit simulation and temperature ranging from 18℃ to 58℃ was investigated by the application of 1H NMR.It was found that not only the strength of hydrogen bonding weakened but also the varying frequency of associative structure and proton exchange speeded up with the temperature increasing.Furthermore,it was testified there was a linear relationship between temperature and the chemical shifts of hydroxyl in related solution,which proved that strong hydrogen bonding predominate in this system.The related concludes partly demonstrated that temperature change could have an effect on the taste of spirit and provided some data support for the process of wine production from spectrum analysis.