In this paper,different extracting methods of Hexanol degradating enzyme from apple were investigated for purpose of comparison,and the best extracting method was obtained.Then crude enzyme extracted with above suitable method was separated and purified for further purification.Results showed that: enzyme extraction with acetone was the best;the SDS-PAGE indicated the electrophoretic bands and molecular weight(MW) of the enzyme obtained through different measures were different from each other.The suitable concentration for ammonium sulphate fractional salting out is 30%~70%,and the Hexanol conversion rate is up to(88.56±0.984 7) % by means of gradient salting out.