Cassava pulp,which has high carbohydrate content,is the waste of cassava starch processing.Non-thermal hydrolysis of cassava pulp by multi-enzyme and different operation procedures were studied.The results showed that the conversion rate of cassava pulp to glucose with fed-batch hydrolysis increased from 53.6% to 72.4% compared with the batch process.The hydrolysate was able to be used for ethanol fermentation without any nutrition supplementation and ethanol concentration reached 24.9 g/L and glucose utilization rate reached 97% at 24 h,as well as ethanol yield 42%,fermentation efficiency 82% and ethanol productivity 1.04 g/(L·h).