In binary organic solvents system,the esterification between ferulic acid and oleyl alchol was investigated,catalyzed by the native Aspergillus niger lipase.The effective factors,such as lipase concentration,temperature and the molar ratio of ferulic acid to oleyl alchol on the esterification were studied.The results indicated that in the isooctane/2-butanone solvents system,under the reaction conditions of Aspergillus niger lipase concentration 0.2 g/mL,temperature 60 ℃,the molar ratio of ferulic acid to oleyl alchol 1∶ 8,substrate concentration of ferulic acid 0.39 mg/mL,and oleyl alcohol 4.3 mg/mL,the conversion rate of esterification was 97.6%.Under the optimal condition in the cyclohexane/2-butanone solvents system which was: Aspergillus niger lipase concentration 0.25 g/mL,temperature 60 ℃,the molar ratio of ferulic acid to oleyl alchol 1∶ 8,the substrate concentrations of ferulic acid at 0.49 mg/mL and oleyl alcohol at 5.37 mg/mL,the conversion rate of esterification was 91.0%.