Canna edulis Ker syrup was firstly used to product succinic acid by Actinobacillus succinogenes CGMCC1593.It was found that the total sugar,corn steep liquid and phosphate significantly improved the production of succinic acid.The Response Surface Methodology(RSM) based on a three-factor Box-Behnken design of experiments was used to build an analytical model and finally employed as a constraint in an obvious optimization process.The optimized parameters were total sugar of 89.66 g/L,corn steep liquid of 38.55 g/L,and phosphate of 4.46 g/L.Under those optimization conditions,it was predicted that the the highest concentation of succinic acid recached 62.92 g/L.After optimization,the succinic acid production increased by 22.7%,and the experimental data basically in line with the predicted values.