functional oligosaccharides are paid more attention in recently years cause their adjustment microecology of intestinal flora,and improvement in healthy.This article reviewed progresses in enzyme preparation of isomalooligosaccharides,trehalose,fructooligosaccharides,galactooligosaccharides,lactosucrose and xylooligosaccharides.The main factors of effect on aim products yield,the applications of enzymes from different sources and the future of enzyme development in functional oligosaccharides production were also described.