The organic selenium and inorganic selenium of selenium enriched peanuts were separated with ultrasonic,centrifuge and dialysis method.The main target for organic selenium is protein and polysaccharide.The content of selenium of the sample was determined by flame atomic absorption spectrometry.The result shows that the extraction of protein was 11.32% and the extraction of polysaccharide was 11.32%.The content of selenium in selenium enriched peanuts was 0.1653 μg/g.Selenoprotein was the major format of selenium in the peanuts,it combined up to73.39% of organic selenium,and 59.89% of the total selenium in the peanuts.The result of antioxidant analysis showed that the crude polysaccharide had longer induction time,higher DPPH free-radical scavenging activity and reducing activity than purified polysaccharide and protein extracts.