A sensitive indirect competitive chemiluminescence enzyme immunoassay(CLEIA)for OTA was developed.The concentration of OTA causing 50% inhibition of binding enzyme marker(IC50) was 112 pg/mL and the detection limit was 2.47 pg/mL,the mean coefficients of variation of within groups and between groups were 7.03,14.7% respectively.The recoveries from rice and wheat samples spiked with 1.5~6 μg/kg of OTA varied between 66.97% and 97.96%,and the OTA antibody showed no cross-reactivity to the else common mycotoxins.OTA in 30 cereal samples were screened by the CLEIA,including 20 rice samples and 10 wheat samples.The results show good coefficient with commercial ELISA kit(R2= 0.9424).The assay was sensitive,fast and accurate,which proved to be suitable for the screening of cereal samples for the presence of OTA.