Traditional purification of chitinase from the fermentation supernatant had some weaknesses such as complicated steps,difficult in controlling and low recovery ratio.Then we took experimental exploration and find a simple,fast and convenient method in the process of chitinase purification.This method may also be used in Batch processing.Detailed method is: fermentation supernatant which containing chitinase was first mixed with colloidal chitin,after centrifugation,the chitinase and the colloidal chitin precipitated and separated with other components in the supernatant.Then the precipitated was washed by distilled water for several times,the precipitate was then resuspended in distilled water and then sterilized at 30 ℃for 72h.After the colloidal chitin was completely hydrolyzed,chitin enzyme was purified and Abstracted.This method requires simple,mild purification conditions,ensuring the purified enzyme maintain its biological activity.SDS-PAGE and colorimetry detecting methods indicated that the recovery rate of chitinase using this method can reach 88.53%.