To establish a method to determine anthocyanin content and composition in red raspberry by HPLC-DAD-ESI-MS.Methods: The analysis was carried on Zorbax SB-C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-5% formic acid-water at the flow-rate of 1.0 mL/min,the detection wavelength was 520 nm and the scan wavelength was from 200 nm to 600 nm.Results and conclusion: The content of gross anthocyanins was 105.69 mg/100 g by HPLC calculated as cyanidin 3-glucoside equivalent.Four anthocyanins in red raspberry were identified by HPLC-DAD-ESI-MS,which are cyanidin 3-sophroside,cyanidin 3-sophroside 5-rhamnoside,cyanidin 3-glucoside and cyanidin 3-rutinoside.Their relative content was 22.05%,13.83%,33.74% and 30.38%,respectively.