A sensitive indirect competitive chemiluminescence enzyme immunoassay(CLEIA) for zearalenone in corn has been developed.The optimized CLEIA gave a limit detection of 0.09 ng/mL and a detection range of 0.104-1.69 ng/mL,with an IC50 of 0.438 1 ng/mL and it was about 3 times more sensitive than that of colorimetric ELISA.It has been validated on cereals samples in terms of precision(intra-and interassay coefficient variations of less than 9.1﹪ and 14.7﹪,respectively) and accuracy(mean recovery 79.6﹪~97.6﹪).These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of ZEN in corn samples.