Food and Fermentation Industries

Determination and Characterisation of Feruloyl Esterase in Wheats and Malts

  • Wang Dan ,
  • Guo Meng-meng ,
  • Du Jin-hua
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Online published: 2011-05-25

Abstract

Taking ethylferulate as substrate,the optimal enzymatic hydrolysis conditions of feruloyl esterase were confirmed as hydrolysis time 100min,the volume ratio of enzymes and substrate 0.75 mL/mL,the pH value of extract 6.2.The study on the characteristic of feruloyl esterase exhibited that the optimal temperature was 60℃,and it had a better thermal stability between 30~35℃,remaining over 80% of enzyme activity after insulation;the optimal pH value was 5.0,and the destruction was relatively weaker,remaining over 80% of enzyme activity after insulation;The activity was inhibited by Cu2+,Zn2+,and strongly activated by EDTA.The activity of feruloyl esterase was determined for five kinds of wheats and malts,and it was found that all of the activity of the enzyme was raised after germination in which the increase of Yan 2415,Lu Mai 21 and Zheng Mai 004 was maximum.

Cite this article

Wang Dan , Guo Meng-meng , Du Jin-hua . Determination and Characterisation of Feruloyl Esterase in Wheats and Malts[J]. Food and Fermentation Industries, 2011 , 37(05) : 181 -185 . DOI: 10.13995/j.cnki.11-1802/ts.2011.05.032

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