Food and Fermentation Industries >

2011 , Vol. 37 >Issue 05: 157 - 159,165

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2011.05.039

Food and Fermentation Industries

The Influence of Membrane's Type and the Pretreatment Methods to the Detection of Staphylococcal Enterotoxin A,B and C in Raw Milk by Lateral Flow Test Strips

  • 林松 ,
  • 何艳玲 ,
  • 王丽哲 ,
  • 赵瑜 ,
  • 袁飞 ,
  • 杨海荣 ,
  • 陈颖
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Online published: 2011-05-25

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Abstract

To establish rapid assays for individual detection of Staphylococal Enterotoxin A,B and C(SEA,SEB,SEC) in raw milk samples,Lateral Flow Immunoassay test strips were studied.The models of nitrocellulosemembrane and the pretreatment methods of raw milk were chosen and optimized,and the membrane of HF09002 and the method of 2 times dilution showed the best result for the field detection of raw milk samples.The Sensitivties of Staphylococcal Enterotoxin A,B and C test strips were 10ng/ mL,4ng/ mL and 8ng/ mL,and there is no observe difference objected that compared with 3M(tm) Tecra(tm) Staph Enterotoxin ID Test method.

Key words： Staph enterotoxin A; B and C; Taw milk; lateral flow immunoassay

Cite this article

林松 , 何艳玲 , 王丽哲 , 赵瑜 , 袁飞 , 杨海荣 , 陈颖 . The Influence of Membrane's Type and the Pretreatment Methods to the Detection of Staphylococcal Enterotoxin A,B and C in Raw Milk by Lateral Flow Test Strips[J]. Food and Fermentation Industries, 2011 , 37(05) : 157 -159,165 . DOI: 10.13995/j.cnki.11-1802/ts.2011.05.039

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