The gene encoding maltogenic amylase was fused with signal peptide gene encoding amylase of Bacillus licheniformis by overlapping PCR.The fused gene(BlMa) was ligated with an expression vector of Bacillus,pHY-P43.B1Ma transforms Bacillus subtilis directly and results in the recombinant plasmid pHY-P43-BlMa.Bacillus subtilis 1A717 which is deficient in amylase was selected as expression host.The recombinant strain was designated as Bacillus subtilis / pHY-P43-BlMa.Enzyme activity assay and SDS-PAGE showed that recombinant maltogenic amylase of B.licheniformis(BlMa enzyme)expressed by B.subtilis / pHY-P43-BlMa was secreted into medium.High Performance Liquid Chromatography(HPLC) identified that major hydrolysate of starch catalyzed by BlMa enzyme was maltose.Culture condition of Bacillus subtilis / pHY-P43-BlMa in flask scale was optimized for production of BlMa enzyme,the optimized medium was: 10 % corn starch,2.5 % cotton seed protein,0.3 %(NH4)2SO4,0.03 % CaCl2 and 0.1 % NaH2PO4.The maximum enzyme activity under optimized condition reached 5.9 U/mL.