Based on desorption effect on total triterpenoids in culture broth of Antrodia camphorata,the adsorption capability and desorption parameters of macroporous resin were studied.The best macroporous resin,XAD-16,has good regeneration ability and the adsorption process is in accordance with Langmuir equation.The optimal separation conditions were as follows: culture broth with pH of 6.0,flow rate of 2 mL/min,100% Ethanol as desorption solution.After separation by macroporous resin,total triterpenoids sample with the purity of 260.54 mg/g was obtained from culture broth of Antrodia camphorata.