The article studied the optimal conditions of removing protein from polysaccharide of Plantago asiatica L.with AB-8 macroporous adsorption resin.The purification conditions were optimized by dynamic adsorption and desorption.The results showed that the optimum conditions were as follows:the concentration of solution was 40 mg/mL with pH of 7.0,the sample loading flow rate was 0.5 mL/min.The elution solvent was 2.5BV distilled water at flow rate of 2mL/min.AB-8 macroporous adsorption resin had the best effect,the protein removing rate was 84.83% and polysaccharide retained rate was 88.32%.