Organic membranes were used to remove cells from deacetylated gellan gum by microfiltration.The performance of different organic membranes and the operating conditions such as feed concentration,temperature,pressure were studied.The optimum conditions were as follows: 3 g/L deacetylated gellan gum,pH 10,65℃,0.1MPa.The results showed that the cell removal and gellan gum recovery were 97% and 84%,respectively by microfiltration using PES membrane with a pore size of 0.45μm.The flux of foul membrane can be well recovered by washing the membrane with 0.75% HCl and 1% NaOH alternately.