Food and Fermentation Industries >

2011 , Vol. 37 >Issue 06: 161 - 164

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2011.06.042

Food and Fermentation Industries

Seven-Plex PCR Detection Method for Genetically Modified Maize

  • Chen Zhen ,
  • Lu Chun-bin ,
  • Yang Meng-jie ,
  • Ma Jun ,
  • Bai Wei-bin ,
  • Wu Xi-yang
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Online published: 2011-06-25

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Abstract

To establish Seven-plex PCR detection system for detecting transgenic components in genetically modified Maize,endogenous gene IVR,exogenous herbicide gene BAR and PAT,exogenous insect resistant gene Cry1Ab,screening gene NPTII,CaMV35S promoter and Nos terminator were chosen for the targeted fragment.7 pairs of primers are designed and the PCR conditions for the multiplex PCR analysis were optimized according to the primer concentration and annealing temperature.The results show that Seven-Plex PCR system can detect endogenous gene and transgenic components with high efficiency and good stability.

Key words： maize; genetically modified components; Seven-Plex PCR detection

Cite this article

Chen Zhen , Lu Chun-bin , Yang Meng-jie , Ma Jun , Bai Wei-bin , Wu Xi-yang . Seven-Plex PCR Detection Method for Genetically Modified Maize[J]. Food and Fermentation Industries, 2011 , 37(06) : 161 -164 . DOI: 10.13995/j.cnki.11-1802/ts.2011.06.042

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