To optimize the extraction of monosaccharides from Jerusalem artichoke residue based on inulinase treatment,the single factor test and the orthogonal test were used.The results showed that the optimum conditions are pH4.6,enzyme dosage 25U/g,extraction temperature 60℃,liquid ratio 1∶ 25,the extraction time 6h.Under these conditions,the average extraction yield of monosaccharide is 82.67%.The hydrolysate contains mainly two kinds of simple sugars,fructose and glucose by TLC analysis.And the contents of two monosaccharides were 135.9mg/g and 84.9mg/g using the DNS method,the ratio of the two sugars was 1.6∶ 1.