After comparing the adsorption and desorption rate of XDA-7,AB-8,D101,DA-201,X-5,XDA-7 was selected as the optimum resin to purify the roselle red pigment.The purifying processing conditions of roselle red pigment was confirmed by the single factor and orthogonal test,and the structure of the roselle red pigment was identified by IR.The optimum absorption conditions were :sample 2mg/mL,the flow rate 1.75mL/min,pH 2.76.The optimum desorption conditions were : the volume of elution 60mL,concentration of eluent 75%,pH of eluent 3.5,elution rate 1.5mL/min.After the purification,the color value of roselle red pigment was 52.6,which was as much as 7 times of unpurified pigment.The structure of the roselle red pigment was identified by IR.The main structure of the pigment was cornflower-glucosides or delphinium-glucosides by IR.