In order to improve the purity of sulforaphen,the separation and purification processes for sulforaphen by macroporous resin adsorbent was studied.SP850 was selected as the best by static adsorption and desorption experiments.The conditions of dynamic adsorption and dynamic elution were also investigated.The biggest volume of SP850 resin adsorption was 20 BV sulforaphen solution and its adsorption curve fit Bohart-Adams model.The optimum technology parameters were : sulforaphen solution volume 20BV,flow rate 5BV /h,50% ethanol for elution,elution volume 20BV,elution velocity 3BV/h.Under the above conditions,the adsorption capacity was 32.83mg/mL resin.The elution field was as high as 91.84%,and the purity of sulforaphen product reached 88.7%.The research results provided a reference for sulforaphen industrial purification.