The species mutualism of lignin-degrading enzymes secretion under co-fermentation in liquid corn-cob medium between white-rot fungi Lentinus edodes,Pleurotus ostreatus and Ganoderma lucidum were studied.The finding demonstrated that: in individual fermentation,the activities of laccase(Lac),lignin peroxidase(LiP) and manganese peroxidase(MnP) of L.edodes all reached the peak values of 1120 u/L,358.2 u/L and 875 u/L in the 10th day;and the activities of Lac,LiP and MnP of P.ostreatus reached the peak values as 3060 u/L,567.8 u/L and 1275 u/L in the 6th day;G.lucidum's Lac active value reached the peak in the 10th day as 2945 u/L,its LiP and MnP active value reached the peak in the 8th day as 428.2 u/L and 975 u/L.Mixed culture of L.edodes and P.ostreatus,P.ostreatus and G.lucidum both can increase the enzymatic active peak value and the peak area.And the co-fermentation of P.ostreatus and G.lucidum has the most obvious effects.In the mixed culture system of P.ostreatus and G.lucidum,the activity of Lac,LiP and MnP all reached the peak values as 11 400 u/L,924.5 u/L and 2200 u/L in the 10th day.The experiment indicated that the mixed culture of different white-rot fungi species had effect on the lignin-degrading enzymes secretion.