In this paper,the cell morphologies of Aureobasidium pullulans G58-2 under the dialysis cultivation,and the optimal cultivation conditions for pullulan production were studied.The results showed that inoculation by dialysis cultivation was better than normal cultivation,OD600 of bacterial suspension was 0.442,the inoculation time 36 h,initial pH7.0,250 mL shake flask containing 50 mL of sucrose medium with 200 r/min.Under these conditions,the highest pullulan yield(4.84 g/100 mL) with the lowest pigment was obtained,and the stability of experiments effectively was improved.