In this preliminary study,the Immunomagnetic Beads Capture-Real time PCR(IMB-RTPCR) method for detecting Salmonella in food was established.The carboxyl magnetic microsphere with a particle diameter of 700 nm was selected as the solid phase carrier.EDC(6 mg/mL)and NHS(5 mg/mL)were used for activation.Subsequently,the 100 μg/mL anti-Salmonella antibody was incubated with the magnetic microsphere at 37℃ for 1.5 h in order to obtain the immunomagnetic bead.The capture efficiency of self-made beads was equivalent to Immunomagnetic beads kit(Dynal)while the former has lower cost.The method has the test limit up to 104 CFU/mL,and it could separate Salmonella effectively up to 104 CFU/mL within 16h.This method is applicable to routine food Salmonella testing.