Frozen abalone visceral were used as materials.The general components of abalone visceral were analyzed.The optimized conditions of ultrasound-assisted glycoprotein extraction was selected by orthogonal analysis on the basis of single factor experiments.The optimized conditions of ultrasound-assisted extraction were as follows: NaCl concentration was 5%,the ratio of material to solvent was 1∶ 9,ultrasonic power was 300 W and ultrasonic time was 40 min.The extraction rate of glycoprotein in the extraction was 69.7mg/g by this method,the extraction ratio increased by 28.5% compared to the tradition method.Furthermore,the glycoproteins were precipitated by(NH4)2SO4 and isolated by DEAE-Cellulose and SephacrylS-300 HR.Results showed that a homogeneous glycoprotein(AGP-Ⅱ) was obtained.The molecular weight of AGP-Ⅱ was estimated to be 73.7 ku by SDS-PAGE.AGP-Ⅱ contained 12.4% total sugar,76.6% protein.