Food and Fermentation Industries >

2011 , Vol. 37 >Issue 09: 36 - 40

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2011.09.047

Food and Fermentation Industries

Phylogenetic Diversity Analyse of Bacteria in Gujing-flavor Liquor Daqu Using Culture Independent Method

  • Tang Bin ,
  • Liu Jin-ying ,
  • Zhou Qing-wu ,
  • Li An-jun ,
  • Wan Chun-huan ,
  • Tang You-hong
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Online published: 2011-09-25

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Abstract

Culture independent method was used to study the diversity of Daqu bacteria.The techniques including culture independent approach,PCR technique and 16S rDNA sequence homology analysis etc.The total DNA directly extracted from Daqu was used as PCR template.Random clones,containing almost full size 16S rDNA sequences(about 1.5 kb long),were sequenced and subjected to an on line similarity search.According to the sequences alignment and through the analysis of its comparability,the bacteria of Daqu were consisted of Lactobacillus,Pantoea,Enterobacter,Klebsiella,Leuconostoc,Erwinias,Pseudomonas and Bacillus licheniformis,which indicated their bacterial diversity.

Key words： Gujing-flavor Liquor Daqu; microbiology diversity; culture independent approach; phylogenetic analysis

Cite this article

Tang Bin , Liu Jin-ying , Zhou Qing-wu , Li An-jun , Wan Chun-huan , Tang You-hong . Phylogenetic Diversity Analyse of Bacteria in Gujing-flavor Liquor Daqu Using Culture Independent Method[J]. Food and Fermentation Industries, 2011 , 37(09) : 36 -40 . DOI: 10.13995/j.cnki.11-1802/ts.2011.09.047

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