The dynamic variation of the accumulation of biomass,Cordyceps polysaccharides,cordycepic acid and cordycepin were compared through fermentation process.The results showed that 70% of Cordyceps polysaccharide,Cordyceps acid and cordycepin was distributed in the fermentation broth.The optimum fermentation period was 12d by considering these three products.The results of 10 L fermentation tank submerged culture test showed that the biomass reached 24.5 mg/mL,which increased 19.86% compared with shaking flask culture,while the Cordyceps acid,CP,cordycepin content were 7.43 mg/mL,2.82 mg/mL,90.73 μg/mL respectively,which increased 8.3%,13.7% and 15.6% compared with shaking flask culture.