Xylose reductase and xylitol dehydrogenase from Candida shehatae amplified by PCR were ligated to yeast expression vector pYES2,to establish recombinant expression vectors pYES2-XYL1 and pYES2-XYL2,respectively. Recombinant expression vector pYES2-XYL1-XYL2 which was acquired by legating XYL1 harboring GAL1 promoter cloned from pYES2-XYL1 to pYES2-XYL2 downstream was transformed to Saccharomyces cerevisiae host strain INVSc1 by electroporation.The ethanol yield was 33.45 g/L under the conditions;initial pH5.5,temperature at 33℃,rotation speed at 50r/min after 150 r/min for 5 h.