Spore-producing and haploid matching(a/α) experiments were carried out to obtain 5 haploids with mating type a,and 7 haploids with mating type a from the parental diploid BY-14.The fermentation capability of the haploid strains were studied,and two haploids(17αand 70a) with high maltose fermentation ability and low glucose effect degree were identified to investigate the fermentation kinetics in maltose-free,glucose-free and mixed LSMLD medium respectively.Both the haploids had short lagging phases of maltose metabolism after glucose exhaustion, which provide a good base for breeding more excellent yeast strain with higher speed of maltose metabolism.