This study isolated main patulin-producing strains from decayed Yang Xin and Lai Yang pears,analyzed and identified their morphological characteristics and ITS sequences.In this paper,patulin-producing strains were isolated from storage pears and test the concentration of patulin produced by HPLC.The fungal ITS region-specific primers were used to do ITS PCR amplification sequencing,through the blast analysis between ITS sequences and GenBank nucleic acid database,finally their biological classifications were identified and their relationships were analyzed.Result: 10 patulin-producing strains were isolated from storage pears,including 1 strains of Fusarium,2 strains of Aspergillus,and the remaining 7 strains of Penicillium.The phylogenetic tree showed that strain P-9 belonged to one group,the other nine strains to another group and they have different levels of the phylogenetic relationships.The main patulin-producing strains are Penicillium,and the P-1 has the strongest ability.