In the paper,carotenoids,amino acids and fatty acids in Sporidiobolus pararoseus JD-2 and Rhodotorula sp.were studied.The total contents of carotenoids in Sporidiobolus pararoseus JD-2 and Rhodotorula sp.were 21.42mg/L and 8.02 mg/L respectively.The main carotenoid in Sporidiobolus pararoseus JD-2 was β-carotene,but the main carotenoid in Rhodotorula sp.was torulen.The isomers of β-carotene in Sporidiobolus pararoseus JD-2 were studied.Among them All-trans β-carotene took up 60% of total β-carotene.A large proportion of cis-forms were 13-cis-β-carotene and 9-cis-β-carotene.Proteins in Sporidiobolus pararoseus JD-2 and Rhodotorula sp.were analyzed.Twenty common amino acids were all found in them.Unsaturated fatty acids took up 74% and 76% of all the fatty acids in Sporidiobolus pararoseus JD-2 and Rhodotorula sp.Carotenoids,proteins,and fatty acids in Sporidiobolus pararoseus JD-2 were all compared with Rhodotorula sp.This paper will form the foundation for the further use of Sporidiobolus pararoseus JD-2 in feed industry.