A comparative analysis of the total flavones content in Panax notoginseng flowers from different areas was performed.The total flavones content in Panax notoginseng flowers was determined at 508nm by ultraviolet spectrophotometric with rutin as the reference substance.Based on the experiment of single factor,the optimum extraction conditions were as follow: ethanol concentration 60%,ratio of liquid to solid 1:25,extraction time 1h.Total flavones in Panax notoginseng flowers from different areas were compared and highest content was 0.73%,the lowest was only 0.43%.The repetition,stability and precision of the method in analyzing the total flavones content are promising.The method can be used to control the quality of Panax notoginseng flowers.