Food and Fermentation Industries >

2013 , Vol. 39 >Issue 01: 36 - 40

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2013.01.010

Food and Fermentation Industries

Screening of high proline yield mutants by rapid mutation using atmospheric and room temperature plasmas

  • Zheng Ming-ying ,
  • Cai You-hua ,
  • Lu Zui-qing ,
  • Yan Jie-neng ,
  • Zhang Xue ,
  • Zhang Chong ,
  • Li He-ping ,
  • Xing Xin-hui
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Online published: 2013-01-25

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Abstract

To enhance the productivity of proline from Corynebacterium acetoacidophilum(originated from glutamic acid producing strain,ATCC-13870),a novel mutation system with atmosphere and room temperature plasmas(ARTP) was used.Under a cell lethality rate of 99%,accompanying with a high throughput screening method using 48-well microplates,16 mutants with changes on growth rates and proline production as compared with wild type were obtained.Experiment results indicated that the proline concentration produced by D3 mutant was increased from 5.47(g/100 mL) of the wild strain to 6.58(g/100 mL) after 48h fermentation.

Key words： atmospheric and room temperature plasmas(ARTP); proline; high throughput screening; mutation

Cite this article

Zheng Ming-ying , Cai You-hua , Lu Zui-qing , Yan Jie-neng , Zhang Xue , Zhang Chong , Li He-ping , Xing Xin-hui . Screening of high proline yield mutants by rapid mutation using atmospheric and room temperature plasmas[J]. Food and Fermentation Industries, 2013 , 39(01) : 36 -40 . DOI: 10.13995/j.cnki.11-1802/ts.2013.01.010

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