In this paper,the reversed phase high performance liquid chromatography(HPLC) method was applied to detect eight kinds of common biogenic amine content in beers.It took the benzoyl chloride as derivative reagent,Agilent Eclipse XDB-C18 as stationary phase,acetonitrile and ammonium acetate solution as mobile phase.Ultraviolet detection wavelength of 254 nm and gradient elution were set.First of all,biogenic amine types and the range of its content were studied.Test results showed that the commercially available beers contained 8 kinds of biogenic amines,but different samples contained different types.And some amines content was too low to be quantified.The products from different manufacturer had different amounts and content.The total content of beer biogenic amine was 4.21-10.59 mg/L.It was too low to harm human health.Then this paper studied the relationship between the original wort concentration,alcohol content and the biogenic amine content.The results indicated that the two factors had some relationship with amine content,though they were relatively minor factors compared with different manufacturers and fermentation conditions.
Luan Guang-hui
,
Liu Chun-feng
,
Li Qi
. The comparison of biogenic amine content in different beers[J]. Food and Fermentation Industries, 2013
, 39(01)
: 176
-180
.
DOI: 10.13995/j.cnki.11-1802/ts.2013.01.030