Food and Fermentation Industries

Purification and antioxidant activity of polysaccharide from Actinidia arguta

  • Xuan Li ,
  • Liu Chang-jiang ,
  • Liu Yang
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Online published: 2013-02-25

Abstract

Actinidia arguta polysaccharide extracted by microwave was purified and the antioxidant activities of different fractions were measured.One water elution fraction and three brine elution fractions were separated using a DEAE anion-exchange column,SephadexG-100.SephadexG-200 column were used for further purification.The results indicated the four fractions were homogeneous and did not contain protein.The results of antioxidant activity indicated Actinidia arguta polysaccharide had definite scavenging effects on DPPH· and ·OH,and the scavenging effects on O-2·were indistinctive;antioxidant activity of brine elution fractions were obviously stronger than water elution fraction;the IC50 scavenging DPPH· and ·OH of 0.1 NaCl elution fraction,0.2 NaCl elution fraction,0.3 NaCl elution fraction and Vc were 0.57 mg/mL,1.61 mg/mL,1.18 mg/mL,0.03 mg/mL and 1.5 mg/mL,5.6 mg/mL,2.7 mg/mL,0.2 mg/mL,respectively;0.1 NaCl elution fraction was the main antioxidant activity component in Actinidia arguta polysaccharide.

Cite this article

Xuan Li , Liu Chang-jiang , Liu Yang . Purification and antioxidant activity of polysaccharide from Actinidia arguta[J]. Food and Fermentation Industries, 2013 , 39(02) : 109 -113 . DOI: 10.13995/j.cnki.11-1802/ts.2013.02.006

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