A new method for determination of trace manganese(Ⅱ) in honey by flame atomic absorption spectrometry combined with cloud point extraction was developed.The cloud point extraction system was based on TritonX-100 as surfactant and 8-Oxyquinoline as complexing agent.The parameters affecting cloud point extraction,including pH,extraction temperature,time,ionic strength,dosage of extraction agent and complexing agent were investigated and optimized.Under the optimum conditions,a good linear relationship was observed for manganese(Ⅱ) in the range of 100-700 ng/mL with the detection limit of 5.74 ng/mL and the relative standard deviations of 1.68%(n=11).The recoveries were in the range of 97.3%-98.6%.The method was rapid,accurate,simple and suitable to apply in the determination of manganese(Ⅱ) in honey.