Food and Fermentation Industries >

2013 , Vol. 39 >Issue 02: 92 - 96

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2013.02.019

Food and Fermentation Industries

Isolation,identification and enzyme purification of a strain producing cold-activity β-galactosidase from marine

  • Cui Ai-ping ,
  • Chi Nai-yu ,
  • Zhang Qing-fang
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Online published: 2013-02-25

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Abstract

A strain CD6 from Huanghai sea mud was identified as Raoultella terrigena according to its morphology,taxonomical properties and molecular characterization.The purified β-galactosidase was obtained through dialysis,ultrafiltration,and column chromatography.Researches on the β-galactosidase showed that its optimal temperature was 25℃ and it was defined as cold-activity enzyme.

Key words： β-galactosidase; identification; purificayion; cold-activity enzyme

Cite this article

Cui Ai-ping , Chi Nai-yu , Zhang Qing-fang . Isolation,identification and enzyme purification of a strain producing cold-activity β-galactosidase from marine[J]. Food and Fermentation Industries, 2013 , 39(02) : 92 -96 . DOI: 10.13995/j.cnki.11-1802/ts.2013.02.019

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