The study was to optimize the alcohol reflux extraction of myricetin from myrica rubra leaves.The selection of extraction factors was investigated on the basis of one-factor-at-a-time(OFAT) experiments.Then,the significance of each factor and interaction analysis of dependent variables and independent variables were studied according to a three-variable,three-level central composite design.The results showed that material/liquid ratio had an extremely significant effect on myricetin yield,volume fraction of alcohol significantly affect myricetin yield,whereas extraction temperature and extraction time had no significant effect on myricetin yield.Under the conditions of : extraction 1.5h at 84.86℃,with 82.09% alcohol assistance and liquid/material ratio of 12.82,the maximum myricetin yield was reached to 1.355%.