In this paper,immobilized enzyme chemiluminescence glucose sensor was made,the sensor can be used for rapid detection of glucose.GOD enzyme column was prepared through the immobilization of GOD with MCM-41 mesoporous molecular sieves and then used in flow injection chemiluminescence analysis system.The optimum condition for GOD column was reaction temperature of 40℃,glucose solution of pH=6.0,velocity of 3 r/min.The liner range of glucose concentration was from 1 to 200 mg/L.The detection limit was 0.2 mg/L.The relative standard deviation(RSD) was 1.8%(n=10),and the recoveries were 97.81%-102.0%.The results showed that the method possessed many advantages such as saving enzyme reagent,simply handling,easy operation,high specificity and rapid determination.There was no significant difference between the determination results obtained through this method and through the standard method.