In order to obtain the best preparation process for tilapia scales collagen peptide zinc chelate,response surface methodology was used to optimize the preparation process,and a mathematical model of peptide zinc chelate yield with the relation of pH,peptide zinc mass ratio,time and temperature was established.The optimal preparation process was as follows: pH 5.0,peptide zinc mass ratio 2.5:1,time 35 min and temperature 54℃.Under the above condition,the predicted value of the peptide zinc chelate yield was 92.79%,and the experimental value was 91.96%,which was no significant difference between the experimental and predicted values.The mathematical model obtained in this experiment will provide a basis for further research of fish scales processing.