Membrane separation technology is an emerging separation technology developed in the late 20th century,besides the function of separation,purification,concentration and refinement,membrane separation has superiority in high efficiency,energy saving,easy operation and simple equipment in contrast with the traditional separation technologies.Nowadays membrane separation has become one of the most important means of modern separation science.In this paper,membrane separation technology is systematically reviewed and the present situation of its application in enzyme solution concentration is also introduced.The key problems limiting the application of membrane separation technology in industrial production of enzyme preparation are discussed,as well as its application in future.