In the present study,the operation parameters including aeration rate and initial glucose concentration during 50-L batch fermentation were optimized to increase the 2-keto-D-gluconic acid(2KGA) productivity from rice starch hydrolysate by Arthrobacter globiformis C224.The kinetic models for describing the cell growth,2KGA production and glucose consumption were proposed by using Logistic/Luedeking-Piret equations and mass balance calculation.Results showed that the 2KGA yield decreased slightly and its productivity lowered significantly when the aeration rate was below 1.5 v·v-1·m-1.The initial glucose concentrations varying from 120.0 g/L to 180.0 g/L were optimal.Under the optimized conditions,the productivities and yields reached to 6.36～6.54 g/(L·h) and 0.96～0.97 g/g,respectively.Those proposed models could fit the predictive values well with the obtained experimental data.The determination coefficients(R2) of all models were over 0.95,which also indicated that theses models could elucidate the 2KGA metabolic characteristics of Arthrobacter globiformis C224.