L-tyrosine,one of the aromatic amino acids,is widely used in food,pharmaceutical and chemical fields.Due to the low ability to accumulate L-tyrosine in natural microbes,the usage of metabolic engineering to modify and optimize global L-tyrosine metabolic pathways has made remarkable achievements in recent years.Several metabolic engineering strategies like removing feedback inhibition,increasing the supply of precursors,blocking competed pathway and regulating the transport system are very effective to improve the yield of L-tyrosine.Moreover,some new technologies such as modular engineering,global transcription machinery engineering and small RNA engineering have raised the L-tyrosine production to a higher level.Metabolic pathway of L-tyrosine in E.coli is a complex metabolic network coordinately controlled by multi-genes and multi-regulations.With the development of biotechnology,re-rational design,artificial synthesis and global optimization is the future development direction of the L-tyrosine metabolic engineering.