A real-time PCR method was developed for the specific,sensitive and rapid detection of ISAV(Infectious salmon anemia virus).A pair of specific primers and a specific probe was designed according to the ISAV gene conserved sequence.The real-time fluorescence PCR probe for detecting ISAV was established.The results showed that this method had good specificity and repeatability,the detection of ISAV minimum copy number was 13.Therefore,the study established the ISAV specific,sensitive and rapid real-time fluorescence PCR probe for fishery products.